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Johnson , L. Oxford Academic. Google Scholar. Cite Cite L. Select Format Select format. Permissions Icon Permissions. Abstract Spermatogenesis is a lengthy process of life involving cell division and cell differentiation by which spermatozoa are produced in seminiferous tubules.
Issue Section:. You do not currently have access to this article. All the alternative splice events that occurred in our sample were identified using replicate multivariate analysis of transcript splicing rMATS 7. Statistical analysis was performed using GraphPad Prism 5 software. The datasets presented in this study can be found in online repositories.
ML and RW designed the project. RW and JZ performed most of the experiments. BZ and XH offered the mouse and analyzed phenotypes.
All authors participated in the manuscript preparation and approved the final manuscript. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article, or claim that may be made by its manufacturer, is not guaranteed or endorsed by the publisher.
At least tubules were counted from two mice for each immunostaining assay. All sections were stained with DAPI to label nucleus. Immunostaining of meiotic chromosome spreads using the antibody against BRCA1. B Quantification of average MLH1 foci per spermatocyte. Supplementary Figure 6 Identification of the enriched spermatogenic cells from testes by western blotting. Anderson, L. Distribution of crossing over on mouse synaptonemal complexes using immunofluorescent localization of MLH1 protein.
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Download references. We acknowledge Dr. Heng-Yu Fan, Dr. Marc Pilon, and Dr. Peter Carlsson for discussions. We sincerely acknowledge Dr. Kui Liu for support in the form of funds VR: and and equipment use at the early stage of this study and for discussions. You can also search for this author in PubMed Google Scholar. Correspondence to Chao Yu. Peer review information: Nature Communications thanks the anonymous reviewer s for their contribution to the peer review of this work. Peer reviewer reports are available.
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